Restoration Strategies Science Plan

Improving Resilience of Submerged Aquatic Vegetation in the Stormwater Treatment Areas

Description/Objective

Submerged aquatic vegetation (SAV) communities are important for Stormwater Treatment Area (STAs) performance as they remove phosphorus (P) from the water column. This occurs primarily by chemical reactions where phosphate coprecipitates with calcium (Ca) as CO2 is removed from the water column during photosynthesis. This coprecipitation is a major mechanism of P removal from the STAs, especially at low P concentrations,  prior to discharge into the Everglades.  

The objectives of this study are to investigate SAV biology, water chemistry, soil chemistry, soil physical characteristics, herbivory, and their interactions to understand SAV species distribution, persistence, colonization, and recovery in STAs.

The study has two phases:

Phase I of this study reviews literature and analysis of historical SAV data [1] to develop a detailed study plan for Phase II.

Phase II of this study evaluates potential stressors of SAV growth and persistence with field surveys and experiments using mesocosms or enclosures to evaluate the effects of marl, muck, or aged muck soils [2]; hydraulic and P loading rates [3, 4]; drying out wetland soils [3]; and herbivory by fish [3].

More Information can be found here:

Detailed Study Plan

Activities Completed and Current Status

Phase I compiled SAV survey datasets between 2000 and 2018 and key data gaps were identified. Literature and historical data of selected STA flow-ways were reviewed. Field monitoring methods were evaluated and optimized. This phase is complete.

Phase II initiated four experiments based on the results of Phase I, three experiments have been completed.

Results

Phase I literature and data analyses found that SAV growth, persistence, and sustainability can be affected by numerous factors [1, 2, 3, 4]. These include: P and nitrogen loading, turbidity, Ca concentrations, water depth, sediment accretion, dry down/reflood events, wind/wave disturbance events, herbivory, and physiological senescence.  

Results of SAV surveys indicate that, 1) SAV standing crop biomass and tissue P content decreases with distance in an STA flow-way, 2) floating aquatic vegetation (FAV) encroachment into SAV areas, followed by herbicide treatment of FAV biomass, may reduce SAV densities and  P removal performance, and 3) extreme environmental events (e.g., high flows, drawdown or cold temperatures) can change SAV community distribution.

Phase II Experiment 1 compared SAV growth on farm muck, aged muck (older muck soils from STAs), and marl soils in mesocosms. Plants grew well with no significant differences among all soils [2]. Plants grown on marl had lower macro-nutrients (P) and micro-nutrients (zinc, copper, and iron) than those grown on farm muck. Plants grown on aged muck had the lowest plant tissue P suggesting reduced nutrient availability as soils in the STA age.  This experiment is complete.

Another mesocosm experiment, experiment 2 investigates the effect of P supply to the water column on Chara (a common SAV species) growth and health.  Chara acquired nutrients relative to this P supply. However less P is removed from the water column when soil P is elevated [4]. Given high external or internal nutrient loads, Chara concentrates its biomass near the water surface, creating anoxic conditions in bottom waters, which could stress the SAV community and reduce P removal efficiency. Samples of bottom water chemistry will be analyzed to examine the effects of anoxic conditions and how they might impact Chararesilience. This experiment is ongoing.

Experiment 3 was a mesocosm study that examined germination and growth rates of SAV on previously dried soils versus soils that remained hydrated [3]. Germination was highest on previously dried soils. Overall SAV growth rates did not vary between soil types. This experiment is complete.

Experiment 4 examined SAV growth under field conditions in enclosures. Shallow rooted SAVs had the highest growth. Deeply rooted plants grew faster in mixed plant communities than monocultures. Exotic nuisance fish (blue tilapia [Oreochromis aureus]) that are found in the STAs were added to the enclosures and significantly reduced SAV growth [3]. This experiment is complete.

References

1. DBE, 2018, Deliverable A1.1 under Agreement No. 4600003883, SAV Resilience Study Work Plan: Studies on Improving the Resilience of SAV in the STAs. Prepared by DB Environmental, Inc.for South Florida Water Management District, West Palm Beach, FL.
2. DBE, 2019, Deliverable A1.5 under Agreement No. 4600003883, SAV Resilience Study Report 4: Studies on Improving the Resilience of SAV in the STAs. Prepared by DB Environmental, Inc.for South Florida Water Management District, West Palm Beach, FL.
3. DBE, 2020, Deliverable A1.5 under Agreement No. 4600003883, SAV Resilience Study Report 4: Studies on Improving the Resilience of SAV in the STAs. Prepared by DB Environmental, Inc.for South Florida Water Management District, West Palm Beach, FL.
4. DBE, 2022, Deliverable A1.6 under Agreement No. 4600004012-WO08R1, SAV Resilience Study Report 4: Studies on Improving the Resilience of SAV in the STAs. Prepared by DB Environmental, Inc.for South Florida Water Management District, West Palm Beach, FL.

Phosphorus Dynamics in the STAs

Description/Objective

This study investigates the factors and mechanisms that affect treatment performance of underperforming Stormwater Treatment Areas (STAs), particularly  key drivers affecting total phosphorus (TP) concentrations at the lower reaches of the treatment flow-ways.  This study has multiple components designed to evaluate water, vegetation, soils, ultra-fine particulates, and microbially-mediated processeses.  These components will help explain factors and processes influencing STA performance in underperforming STA cells, provide comparisons to these components in well-performing STAs and will help refine and/or improve management options to reduce total phosphorus (TP) discharge concentrations from the STAs.

More Information can be found here:

Study Plan Summary

Activities and Current Status

The study began in January 2021.

Flow-way Water Quality Assessment: Phosphorus (P) cycling is being evaluated at water quality transects in six different STA Flow-ways (FWs): STA-1E Central FW, STA-1E Eastern FW, STA-2 FW 3, STA-2 FW 4, STA-3/4 Central FW, and STA-5/6 FW 1.  Year 1 of monitoring is complete. Two years remain, with the goal of sampling in 2 flow-ways each year, for two 4-month periods to cover the wet (high flow) and dry (low-no flow) seasons. This effort is supplemented with event-based spatial monitoring of water quality, vegetation, and soil conditions in the study FWs under high and no flow conditions. An historical analysis of the monitoring data collected in these flow-ways has been completed [1]. This historical analysis along with the current monitoring will be used to evaluate factors and mechanisms that affect treatment performance of underperforming STAs, particularly those that are key drivers at the lower reaches of the treatment flow-ways. These factors will primarily focus on internal loading as it relates to the interactions of P in the vegetation and soils with the water column. 

Soil P Characterization: Soil cores were collected from all the study FWs and separated into floc, recently accreted soil (RAS), and pre-STA soil for basic soil characterization, P sorption and desorption, and P fractionation [2]. This information will provide an understanding of the STA P performance because P that accumulates in the STAs can be resuspended into the water column from floc and soils. The remobilized P can be transported along the flow path, increasing enrichment of P in soils of the STA treatment cells and affecting TP concentrations to the STA discharge water.

Seasonal Assessment of Biological Indicators: The use of biogeochemical measurements to quantify STA P storages, forms and bioavailability will be evaluated.Relative shifts in nutrient limitation at the different regions of selected well performing and underperforming STA flow-ways will be compared. Water-extractable nutrients, various enzyme activities, microbial biomass nutrients, anaerobic respiration, potential organic P hydrolysis, and organic N and P mineralization will be measured.  This study will begin in the 2022 wet season.

Evaluation of Ultra-Fine Particulates: Particulates <0.45 μm in size could be a significant portion of P in STA discharge water. These particulates will be measured from water samples at inflow and outflow regions of both well performing and underperforming STAs. Floc will be collected and examined for these particulates as well. This study component will examine if these ultra-fine particulates vary with plant type (e.g. EAV vs SAV), potentially affecting outflow TP concentrations. The ability of these particulates to sequester nutrients will also be evaluated.   

Reports and Publications

1. DBE Environmental. 2022 Phosphorus Dynamics in the Stormwater Treatment Areas. Deliverable 6b Year 1 Quarter 4 Annual Report, WO 4600004012-WO09. Submitted to the South Florida Water Management District.
2. University of Florida. 2022. Phosphorus Dynamics in the Everglades Stormwater Treatment Areas:  Evaluation of Biogeochemical Factors and Processes. Draft First Annual Report, WO 4600004016-WO04. Submitted to the South Florida Water Management District.

The Effect of Vertical Advective Transport (Positive Seepage) on Total Phosphorus Concentrations in the STAs

Description/Objective

The purpose of the Advection study is to quantify the relative magnitude of vertical advective transport (positive seepage) of total phosphorus (TP) across the soil-water interface of Stormwater Treatment Area (STA) cells. The study focuses on outflow STA cells to determine if the internal TP load from positive seepage into the water column affects the low TP concentrations in these cells. In addition, the study evaluates management alternatives that may reduce or eliminate the positive seepage. The work will be completed in four phases:

Phase I is a feasibility study to examine if advection can have a significant influence on TP discharge concentration from the STAs. This phase includes three tasks:

1. Literature review – to determine if seepage is a measureable factor of wetland water budgets and TP concentrations.
2. Historical data analysis and water budgets –  compile flow, rainfall, and evaporation data to develop water budgets for a select number of STA outflow cells. These will be analyzed to determine if seepage can be measured indirectly using water levels and conservative tracers. Evaluate water levels within and among surrounding STA cells to determine if differences and/or levels affect TP concentrations, which may indicate a seepage effect.
3. Simple seepage model - A simple numerical model that simulates the hydrology and TP concentrations of an STA outflow cell will be constructed. A positive seepage and associated TP concentration (upward advective flow and TP load) will be added and a sensitivity analysis conducted to determine the effects of seepage on TP concentration in the simulated STA cell.

STOP/GO – if Phase I determines that seepage can significantly affect STA cell water TP concentrations, then Phase II may begin.

Phase II is a pilot study to determine if field sampling can actually measure the effects of seepage on outflow cells of STAs, and if this seepage can be a source of TP that changes the TP concentration within the cell. This phase includes three tasks:

1. Three seepage meters and three pieziometers will be deployed in one outflow STA cell.
2. Seepage, water depth, groundwater pressure and TP concentration will be measured over the course of weeks to a month.
3. Seepage and associated TP concentrations will be evaluated to determine their effects on overlying water quality.

STOP/GO – if Phase II finds measureable seepage, associated water quality and influence on the overlying water column TP concentrations, then Phase III may begin.

Phase III will expand on the Pilot study to determine if seepage affects the outflow TP concentrations in underperforming and well-performing flow-ways. This phase includes three tasks:

1. Five sets of piezieometers and seepage meters will be deployed into outflow cells of two well-performing (annual flow weighted mean [FWM] outflow TP concentration is consistently less than 20 µg P L‑1)  and two underperforming STA flow-ways (annual FWM outflow TP concentration is consistently greater than 20 µg P L‑1).  
2. Measurements will be collected over one wet and one dry season.
3. The effect of seepage on water column TP in the STA cells will be evaluated.

STOP/GO – if Phase III finds measureable seepage, associated water quality and influence on the overlying water column TP concentrations, then Phase IV may begin.

Phase IV will use the results from the previous three phases to develop options that may be used to reduce the effects of seepage on TP concentration in flow-way outflow. This phase includes two Tasks:

1. Information from the previous phases of this study will be considered in the development of options to reduce advective loading to STA outflows.
2. Candidate outflow cells will be identified to manage for advective loadings based on hydrology, location, and outflow TP concentration.

More information can be found here:

Detailed Study Plan

Activities Completed and Current Status

The detailed study plan has been drafted and a statement of work is complete.

The workorder to accomplish Phase I of this study has been executed.

A work plan has been written.

A draft literature review is under review.

Assess Feasibility and Benefits of Consolidating Accrued Marl in the Everglades Stormwater Treatment Areas

Description/Objective

Marl is a significant component of the soils in submerged aquatic vegetation (SAV) dominated systems. It is produced when carbon dioxide is removed from the water by photosynthetic activity of both algae and aquatic macrophytes. This activity shifts the bicarbonate/carbonate equilibrium towards carbonate, and in the abundance of calcium, calcium carbonate (marl) forms.  Some STA soils are physically unstable, allowing rooted macrophytes to be dislodged by wind/wave energy, while resuspension, bioturbation, and movement of unconsolidated marl material can increase water column turbidity and discharge total phosphorus (TP) concentrations. To achieve the Water Quality Based Effluent Limit (WQBEL), cost-effective management strategies to control internal loading of phosphorus (P) to the water column are essential. This study evaluates consolidating marl primarily with organic matter addition. If consolidation of marl can be achieved, P storage can be increased, internal P loading can be reduced by consolidating soils and reducing persistent particulate P in suspension. This approach may improve soil conditions and mitigate soil P release when STA water level drawdown is required for construction or other management efforts. If this consolidation method works, it will reduce water column TP concentrations in the lower reaches of the Everglades STAs.

The study has 3 phases with STOP/GO decisions at the conclusion of Phase I and Phase II.

Phase I includes a literature review and three sequential benchtop mesocosm-scale experiments. The first experiment evaluates the physical stability and aggregation of unconsolidated marl soils from SAV-dominated areas of STA cells/flow-ways. The second experiment compares the stability of consolidated marl with and without organic matter inputs and examines short-term water column P dynamics following resuspension of marl soils. The third experiment assesses the physical and chemical stability of soil TP in consolidated marl soils following rehydration. If Phase I results demonstrate technical feasibility and benefits of consolidating marl, the study may move to Phase II. This phase is ongoing.

Phase II consists of mesocosm-scale studies to compare consolidated marl to amorphous marl and limerock in reducing water column P concentrations.  If significant water quality benefits from consolidated marl soils are found in Phase II, the study may proceed to Phase III. This phase is ongoing.

Phase III involves a cost-benefit analysis of the different methods to improve marl aggregation and reduce TP concentrations that discharge from the STAs to levels that meet the WQBEL.  A comprehensive analysis of experimental data will be conducted and written up as a technical document with recommendations.  This phase has not yet started.

More Information can be found here: 

Project Work Plan

Activities Completed and Current Status

The study was initiated in June 2021.

Phase I literature review [1] and a work plan [2] are complete.

Experiment 1 collected marl soils from multiple SAV-dominated sites and evaluated for physical stability and aggregation [3]. One soil collected from an SAV sight was found to be the least stable, resulting in high water column turbidity and TP concentration and was selected for Experiment 2, the organic matter amendment study. This experiment is complete.

Experiment 2 compares physical stability of organically amended and unamended dried soils. The organic amendments being evaluated include low-TP materials (dead cattail leaves, sugarcane bagasse, and humic soil) applied at 10% and 25% by volume, that may increase soil aggregation and reduce turbidity and water column TP upon suspension.  This experiment is ongoing.

Experiment 3 will evaluate the chemical stability of soil TP in the amended and unamended soils if the first two experiments show increased physical stability and consolidation of marl soils. This experiment will examine the duration of such benefits, the effects on soil P release over time, and the chemical stability of soil TP in the amended and unamended soils. This experiment has not yet begun.

Results

Experiment 1 evaluated the physical stability of seven SAV marl and three emergent aquatic vegetation (EAV) soils by measuring water column turbidity and TP concentrations after controlled laboratory resuspension [3]. This experiment tested the hypothesis that overlying water-column turbidity and particulate P (PP) concentration are inversely related to the organic matter (OM) content of the soil. If true, then marl soils that are low in OM may be more susceptible to resuspension than other soils, and an OM amendment may enhance physical stability. 

Reports

1. DBE, 2021a. STA Marl Literature Review (Agreement 4600004012-WO10; Deliverable 2b; submitted 2021-09-01), Assess Feasibility and Benefits of Consolidating Accrued Marl in the Everglades Stormwater Treatment Areas (STAs). Prepared for SFWMD, West Palm Beach, FL.
2. DBE, 2021b. Project Work Plan. Assess Feasibility and Benefits of Consolidating Accrued Marl in the Everglades Stormwater Treatment Areas (STAs) (Agreement 460004012-WO10; Deliverable 3B; submitted 2021-11-18). Prepared for SFWMD, West Palm Beach, FL.
3. DBE, 2022.  Letter Activity Report 1. Assess Feasibility and Benefits of Consolidating Accrued Marl in the Everglades Stormwater Treatment Areas (STAs) (Agreement 460004012-WO10; Deliverable 4A; submitted 2022-03-01). Prepared for SFWMD, West Palm Beach, FL.

Sustainable Landscape and Treatment in a Stormwater Treatment Area Study

Description/Objective

The purpose of the Landscape Study is to measure transport and dispersion of salt and phosphorus within a cattail community in a controlled (flume) environment to determine the flow volumes and velocities that optimize mixing based on water depth and plant densities. The effects of constant flow and pulsed flow on transport and dispersion also will be measured.

The work will be completed in two phases:

Phase I will install flumes and conduct benchmark studies in four tasks (ongoing):  

1. Installation of one straight and one V-shaped flume in STA-1W South Test Cell 13. This task is complete.
2. “No flow” tests – the flumes will be filled with water and the total phosphorus (TP) concentrations will be monitored to determine the amount of time the water needs to stay in the flume to remove the maximum amount of TP. This task is ongoing.
3. Steady state flow – when flow in equals flow out a specified amount of salt and phosphorus will be added and measured along the flumes pathway to determine mixing and removal, respectively. This task is ongoing.
4. Wave Tests – water inflow will be pulsed, creating waves within the flumes to measure resistance of flow within the flume. Added salt and TP will be measured to determine mixing and removal. This task is ongoing.

STOP/GO – if Phase I is sucessfully completed, Phase II may commence

Phase II will be a Full Landscape Study to include three tasks: Cattail will be planted in the flumes followed by an initial grow in period.

1. Tests 2 through 4 described in Phase I will be repeated at 3, 6, and 15 months after cattail are planted. 
2. Analyses will provide operational guidelines for flow deliveries for optimal mixing and TP reduction in STA flow-ways based on water levels and plant densities.

More information can be found here:

Statement of Work

Activities Completed and Current Status

The Work Order for Phase I of the study has been executed.

A flume installation plan has been received and the flume has been installed.

A draft workplan has been written and is being finalized.

Biomarker Study

Description/Objective

The stormwater treatment areas (STAs) were created to decrease phosphorus (P) inputs into the Everglades. Phosphorus can come in many forms, some of which is associated with particles (particulate P) and organic matter (organic P). These forms of P may be stored in the soils and plants of the STA, consumed by organisms like fish and microorganisms and then recycled into other P forms (internal cycling), or exported to the Everglades. However, we do not yet know all the conditions (drivers) that control the fate of P in the STAs.

A Pilot study indicated that this approach could provide information on P sources and sinks within the STA. This study will measure the forms of P in the STAs, where they may originate, and what factors (light, microbial decomposition) may affect them in the STAs.

This study includes three phases:

• Phase 1: Characterization of STA source waters
• Phase 2: Identification of fish biomarkers
• Phase 3: Experiment to test mechanisms of P turnover in the STAs

More Information can be found here:

Work Plan

Activities completed and current status

The project was initiated in September 2020, work has been begun for all three phases:

Phase 1. Characterization of STA source waters. Source water samples have been collected from eight locations of source water to the STAs and are currently being analyzed at the University of Florida for various organic components (such as amino acids, lignin, and sterols) as well as molecular weight, Xray absorbance, size fraction and P speciation.  These analyses may help to identify the degradability and origin of the P molecules.

Phase 2. Identification of fish biomarkers. In collaboration with researchers at Florida International University, samples from fish excretion studies have been collected and are being analyzed to identify fish excretion “fingerprints.” These fingerprints will assist to determine the contribution of fish excretion to internal P cycling within the STAs.

Phase 3. Experiment to test mechanisms of P turnover in the STAs. This experiment will be conducted in late spring/early summer of 2021 and will evaluate how light and microbial decomposition influence P turnover in the STAs.

Reports and Publications

1. Morrison ES, Shields MR, Bianchi TS, Liu Y, Newman S, Tolic N, Chu RK. 2019. Multiple biomarkers highlight the importance of water column processes in treatment wetland organic matter cycling. Water Res 168: 115153. Elsevier Ltd. doi: 10.1016/j.watres.2019.115153

Data Integration Study

Description/Objective

The purpose of the Data Integration and Analysis Study (Data Integration Study) is to synthesize and summarize results from the Restoration Strategies Science Plan (RSSP) Studies and other STA scientific activities. This synthesis will provide a comprehensive understanding of phosphorus (P) dynamics within the Everglades Stormwater Treatment Area (STA) that affects P retention within these constructed wetlands. A final report, Synthesis, Guidance and Knowledge Gaps of the RSSP Studies will summarize the RSSP, establish guidance for STA management to support achievement of the Water Quality Based Effluent Limit (WQBEL), and consider gaps in knowledge that could be evaluated in the future to improve STA P retention.

The study includes five tasks: (1) review and summarize results from Science Plan reports and publications as well as other relevant STA reports, (2) compile, review, and analyze STA and Science Plan study nutrient, biological, and ancillary data, (3) develop STA P models (empirical and deterministic) to support understanding of P dynamics in the outflow regions of the STAs, (4) determine missing relevant information and methods through a data gap analysis, and (5) develop a guidance document to provide potential STA design, operation, and management strategies to support achievement of the WQBEL at the STA outflows.

More information can be found here:

Detailed Study Plan

Activities, Current Status and Results

The tasks have been carried out through a number of work orders and internal work products

1. Data analyses comparing plant P and soil P and TP concentrations in water of outflow cells (Complete; Task 1). This analysis of outflow STA cells found that the lowest water column TP concentration occurred when moderate to high densities of musk grass (Chara spp.) were present. Relationships of plant and soil P content to water column TP concentration were not as strong, however in studies using mesocosms (typically 1 meter diameter containers that include soils, plants, and inflowing and outflowing STA water) soil and plant tissue P content was related to outflow TP concentration [1].
2. Microbial research review (Complete; Task 1): the microbial research in STAs with specific focus on periphyton (attached algae), processes, biomass, and enzyme activity was reviewed. Reduced mineralization in soils and enhanced enzyme activity in water column promoted lower TP outflow concentrations [2].

3. Historical review of flow-way performance (Complete; Task 2): Data compiled for each flow-way were analyzed over the period of record to determine annual flow-weighted mean outflow P concentrations in relation to major disturbances (dry out/reflood, loss of vegetation, construction) and phosphorus loading rate (P load/m2/yr). Outflow P concentrations were higher in years with disturbances than in years without disturbance. During years with no disturbance in the previous 3 years and when PLR was less than 1.3 g/m2/yr, outflow P concentrations were consistently less than 20 µg/L. When PLR was greater than 1.3 g/m2/yr outflow P concentrations ranged above 20 µg/L. [3,4].

4. STA evaluation (Complete; Task 2): Monthly observed data for each STA for the period of record was compiled and this data was used to develop statistical structural equation models [SEMs]. These models were used to determine if outflow TP concentrations were related to any of the 81 + monthly measurements taken in the STAs [5,6]. The SEMs varied among the STAs suggesting that strategies to enhance P retention will vary and may be unique among the STAs. One factor that was consistent among all SEMs was that TP concentrations in the outflow have declined over time.

5. Flow-way loads, flows, and ancillary data (Ongoing; Task 2): Monthly inflow and outflow loadings have been summarized by flow-way to evaluate their performance separately and collectively. This data set will be useful for current and future modeling and management efforts.

6. Biogeochemical and Comprehensive Aquatic System models (Ongoing; Task 3, 4): Phase I reviewed the literature and compiled STA data for STA-2 FW1 to determine the direct interaction of significant P components within the STAs and develop conceptual biogeochemical and CASM models that represent P cycling in the STAs [7]. Phase II developed simulation models that include mathematical formulations of interactions of the P components [8], these models were enhanced further in Phase III [9, 10]. Phase III is ongoing and will verify and validate model results. Once completed sensitivity analyses will indicate interactions that affect P retention and could lead to management options to enhance P retention.

7. Fire Project review (Ongoing; Tasks 1 and 2): The Fire Project, a 6- ear study, was undertaken to evaluate accelerated (by fire) and natural recovery of a wetland affected by high nutrient surface water loading that resulted in a community shift from historic ridge and slough to cattail-dominated marshes. This review will develop a technical memorandum and apply knowledge gained from the Fire Project to improve performance and management of the STAs to enhance P retention.

8. Synthesis, Guidance and Knowledge Gaps of the RSSP Studies (Tasks 1-5): under development.

1. DB Environmental. 2020. Data Integration and Analyses: Water, Vegetation and Soil Relationships in the Everglades Stormwater Treatment Areas. Prepared for South Florida Water Management District and Everglades Agricultural Area Environmental Protection District in fulfillment of Task A4.4 for Agreement No. 4600004075 on Inter-Agency Agreement to Conduct Scientific Studies Relevant to the Stormwater Treatment Area. Revised November 2020.

2. DB Environmental. 2021. Microbial Processes Affecting Phophorus Removal and Retention in the Everglades Stormwater Treatment Areas. Submitted to South Florida Water Management District in fulfillment of Task 5a. Work Order 4500004012-WO05.

3. DB Environmental, I. 2021. Phosphorus Dynamics in the Stormwater Treatment Areas Annual Report Deliverable 6a: in partial fulfilment of Work Order 4600004012-WO09. Rockledge, Fl

4. Jerauld, M., Vaccare, J., Evans, L., Kruizenga, G., Colios, S., Inglett, P., Judy, J., Wright, A., Subedi, P., King, J., Diaz, O., Wilson, J., Herteux, C. 2024. P Reduction Dynamics IN STA-1E, STA-2, STA-3/4, and STA-5/6 (P Dynamics Study) in: James R.T., Piccone T., King J., Mason S., Bornhoeft S., eds. Chapter 5C: Restoration Strategies Science Plan South Florida Environmental Report. West Palm Beach, FL: South Florida Water Management District.

5. Baser, B. , R. Reddy, J. Hu., R. James, M. Chimney, H. Chen, O. Villapando, J. King, and S. Newman 2021. An Exploratory Analysis of the Everglades Stormwater Treatment Areas Using Structural Equation Models. Submitted to the South Florida Water Management District in fullfillment of Task 4 of Work Order 4600004016-WO05.

6. Hu, J and R. James. 2021. Data Set Compilation for an Exploratory Analysis of the Everglades Stormwater Treatment Areas Using Structural Equation Models. Submitted to the South Florida Water Management District in partial fullfillment of Task 4 of Work Order 4600004016-WO05.

7. Coastal Ecosystems Analyses, Cardno Inc., ESA. 2023. Data Integration and AnalysisvPhase I: Development of Simulation Models for Everglades Stormwater Treatment Areas (STAs) Matrix Development and STA Model Documents. Submitted to South Florida Water Management District in fullfillment of Task 2 of Work Order 4600004014-WO05.

8. Coastal Ecosystems Analyses. 2022. STA Modeling Phase II: Data Integration and Analysis. Submitted to South Florida Water Management District in fulfillment of Tasks 4.4., 4.5, and 4.6 of Work Order 4600004014-WO05 subcontracted to CEA by Ecosystem Science Associates.

9. Coastal Ecosystems Inc. 2023. STA Modeling Phase III: Data Integration and Analysis Contract 4600004014-WO05 to Environmental Science Associates Phase III: Development of Simulation Models for Everglades Stormwater Treatment Areas (STAs) Task 8.3 Test Model Simulations

10. Bartell, S.M.; Woodard, C. 2023. STA Modeling Phase III: Data Integration and Analysis Contract 600004014-WO05R5 with Environmental Science Associates Task 9.1.3 CASM Update and Steady-state Modeling

Use of Soil Amendments or Soil Management to Control Phosphorus Flux

Description/Objective

Stormwater Treatment Areas (STAs) remove phosphorus (P) from agricultural and urban runoff before discharging into the Everglades and have been constructed to a large extent on former agricultural lands. Once the STAs are flooded, P in the soils can be released (flux) into the water column. This study was intended to screen methods that could reduce P flux from the flooded soil by application of soil amendments and/or management techniques. Effective methods may be useful at appropriate locations within the STAs to reduce outflow P concentrations.

The original study plan included three phases:

• Phase I – Review existing information on soil amendments and/or management techniques applicable to wetlands
• Phase II – Conduct small-scale experiments to screen soil amendments or management techniques identified in Phase I
• Phase III – Conduct large-scale field trials of the most promising technologies identified in Phases I and II

More Information can be found here:

Detailed Study Plan

Activities Completed and Current Status

Phase I:  Technologies to reduce soil-P flux in wetlands or lakes were reviewed from the literature and past SFWMD projects. The technologies were evaluated for their potential to scale up to field trials in the STAs [1]. This Phase is complete.

Phase II: Based on what was learned in Phase I about the P sorption capabilities of various materials and the limitations of transferring laboratory results to the field, coupled with the SFWMD’s experience testing soil amendments, it was decided that there was no need to conduct further small-scale experiments with multiple soil amendments. In addition, based on consideration of the uncertainties of long-term treatment effectiveness of soil amendments, potential negative effects on STA operations, high costs to implement a full-scale experiment in the STAs and potential negative effects of soil amendments on the downstream Everglades flora and fauna, this Phase was cancelled.

Phase III: Ongoing. The scope of this phase was scaled back to include only an evaluation of the efficacy of soil management, specifically soil inversion in the STA-1W Expansion Area (EA) #1.

Results
A comprehensive review of available technologies and soil amendments indicated that many potentially could lower P flux from flooded soils [1]. However, due to the uncertainties listed above, all further evaluations were cancelled, with one exception: soil inversion (i.e., soil management without amendments).

Soils in the STA-1W EA #1 Cell 7 were inverted as a remedial action to bury high copper concentrations that resulted from past agricultural practices. Soils in the adjacent Cell 8 were not inverted and are considered to be a control. Prior to flooding these cells, soils were collected and incubated to determine the amount of soluble P that could be released [2].  Phosphorus release from inverted soils was less than non-inverted soils. All STA-1W EA #1 cells have been flooded and the facility began treating water on a restricted basis in late June 2021. Weekly monitoring of P concentrations at Cell 7 and 8 inflow and outflow sites began in July 2021. Surveys of these wetland cells have shown similar soil TP concentrations, overlapping TP water concentrations, and differences in the type and distribution of plants [3, 4].

Reports and Publications

1. Chimney, M., 2015, Phase I Summary Report for the Use of Soil Amendments/ Management to Control P Flux Study. Technical Publication WR-2015-006.  South Florida Water Management District, West Palm Beach, FL.
2. Josan, M., B. Taylor, and K. Grace, 2019, Appendix 5C-3: Use of Soil Inversion to Control Phosphorus Flux. In: 2019 South Florida Environmental Report – Volume I. South Florida Water Management District, West Palm Beach, FL.
3. DB Environmental. 2021.Deliverable 2.4 Soil Management Study – Water Sampling And SAV Survey Report 4, The Use Of Soil Management To Control Phosphorus Flux, November 22, 2021, submitted to South Florida Water Management District, West Palm Beach, FL.
4. Chimney, M. 2022. Use of Soil Amendments/Management to Control Phosphorus Flux (Soil Management Study). In Chapter 5C: Restoration Strategies Science Plan (James, R. T., C. Armstrong, J. King, S. Mason, T. Piccone and O. Villapando, eds) in 2022 South Florida Environmental Report – Volume I.

Evaluate Phosphorus Sources, Forms, Flux and Transformation Processes in the Everglades Stormwater Treatment Areas

Description/Objective

To understand how Phosphorus (P) is processed and removed within well performing Stormwater Treatment Areas (STAs), this multi-component study evaluated mechanisms and factors affecting P reduction from the water column particularly in the outflow regions of the STAs. The study measured flow-way (FW) water quality, internal phosphorus loads, soil, microbial enzymes, vegetation, particulate transport and settling, fauna, and organic P speciation. In addition, these data were synthesized to explain factors and processes influencing STA performance and will serve as the basis to develop or improve management options to reduce total P (TP) discharge concentrations from the STAs.

More Information can be found here:

Detailed Study Plan

Activities Completed, Results and Current Status

The Study was completed in 2019

Vegetation type, depth in the profile and distance from the inflow affected P sorption and desorption. The floc and RAS were more reactive than underlying soils. SAV-dominated sites generally exhibited a greater potential to retain P at low surface water P concentrations, than EAV-dominated sites.  No trend was found for P sorption or desorption from the inflow to the outflow regions. P desorption tended to be higher for EAV than SAV-dominated sites. P sorption was strongly related to extractable and total Fe and Al for EAV-dominated FWs, and Ca and Mg in SAV-dominated FWs.

EAV in STA-2 had higher concentrations of P, N and C in plant material compared to SAV.  Of the SAV species, Chara had the highest nutrient storages of all species.  EAV biomass and performance were consistent throughout the study in FW 1 while FW 3 experienced a decline in performance following the significant loss of SAV. 

• Flow-way Water Quality Assessment: P cycling in three well-performing STA FWs was evaluated under high, moderate, low and no flow conditions.  TP concentrations were high at the inflow region--consisting primarily of inorganic phosphate--and low at the outflow region--consisting primarily of particulate P (PP) and dissolved organic P (DOP). These results were consistent for all flow conditions in all FWs. More P was removed from the water column of emergent aquatic vegetation (EAV) regions than submerged aquatic vegetation (SAV) regions. In SAV regions, TP increased under no flow condition--primarily as particulate P (PP)--which could be from internal loads caused by periphyton sloughing, litter fragmentation, or bioturbation. P concentrations were slightly higher in SAV systems than in EAV systems under no flow condition.   
• Flux Measurements: P concentrations in field chambers increased during no flow periods, indicating internal P loading from vegetation mining of soils and turnover. This increasing P trend, evaluated with a computer model, suggested that internal loading was a significant contributor to the P cycle. Internal loading was highest at inflow regions and lowest in outflow regions of the FWs.
• Soil P Characterization: P concentrations in floc and recently accreted soils (RAS) declined from higher values at the inflow regions to low values at the outflow regions. Phosphorus in floc and RAS was higher and more spatially extensive in EAV than SAV areas of the STAs. The decreasing trend of P from inflow to outflow resulted in increasing trend of N:P ratio from inflow to outflow. P was primarily in an organic form in EAV and inorganic mineral form in SAV areas. P accretion rate was higher in SAV than in EAV due to accumulation of material high in inorganic matter.
• Microbial Enzymatic Patterns: Within the STA flowways, the microbial communities play an important role in nutrient cycling, especially when nutrient limiting conditions exist. Required nutrients (i.e., C, N, or P) can be released from the dissolved organic compounds by enzymes produced by the microbes. Enzyme activity--a measure of microbial community activity--was affected by flow conditions among each component and differing responses were observed along the flowways and within the vegetation communities. The level of enzyme activity was highest in the periphyton and lowest in the surface water. Enzyme activity in the surface water during flow conditions, was often undetectable.  With regard to enzyme response and nutrient conditions, flow resulted in more N-limited conditions in the EAV areas for floc and more P-limited conditions in the SAV areas. In the periphyton, C-limited conditions increased during flow in the EAV areas, but an opposite effect was observed in the SAV areas. In both plant communities, N-limiting conditions in the periphyton was greatest during no flow periods. In both the floc and periphyton, greatest P-limiting conditions were observed at the outflow locations. These variable responses indicate that flow conditions have a complex effect on the microbial responses within each component and that nutrient limiting conditions change along the flowways and within the major components.
• Vegetation Assessments: Satellite images of STA areas in wet and dry seasons detected differences in SAV and EAV spectral signatures. SAV areas dominated by a single species, specifically Chara, could be accurately identified. Mixed SAV species beds were more difficult to distinguish. When cross-validated with ground observations, the spectral analysis correctly classified 96% of reference samples.
• Particulate Transport and Settling: Water velocities in the STAs are affected by gate operations, wind, and distance to remnant agricultural canals and ditches, with highest velocities near inflow and outflow structures and remnant canals/ditches. Inflow regions had the highest observed sediment settling/resuspension and net accumulation rates. Measured net P accumulation rates into floc were similar in magnitude to net P storage estimated from mass balance for STA-2 FW 3.  STA-3/4 Cell 3B had lower storage rates, which is attributed to lower accumulation, higher erosion, and/or more spatial variability at this cell’s inflow regions. At relatively low and stable water velocities (< 0.5 cm/s), water column particulates likely originate from sloughed SAV biomass rather than sediments and are caused by wave related stress rather than flow driven stress. PP resuspension and settling measurements in SAV areas are approximately 10 times higher than total P loading and accumulation rates derived from mass balance. Therefore, the potential for large swings in P storage or loss may be expected depending on the factors that drive these fluxes. 
• Fauna Study: STA fish nutrient content and nutrient ratios were similar to values reported for enriched areas of Water Conservation Area 2A. Fish tissues contained an estimated 1 metric ton of TP and 3.7 metric tons of TN in STA-2 Cells 3, 4, 5, and 6. Because of the substantial fish population, further analyses on the contribution of fauna to the water column P in the outflow regions of STAs is being investigated as a separate Fauna Study.
• Organic P Speciation: Biomarkers (lignin phenols, amino acids, and pigments) as a measure of organic matter (OM) quality were compared between STA-3/4 and a reference wetland WCA-2A. More vascular plant-based OM was found in EAV areas, and more algal-based OM was found in SAV areas. OM was fresher and more degradable in treatment SAV sites than reference wetland sites. Other than dense cattail areas, water column particulate OM in SAV areas was primarily from microbial sources. Amino acid degradation indices and organic P were positively correlated with bacterial amino acid biomarkers, suggesting microbial abundance was associated with “fresher” OM. This multi-biomarker approach may evaluate the relative “freshness” of OM pools, identify sources of OM, and provide relative measures of water column processes in P cycling in STAs. Due to the significant results further analysis is being conducted under a separate Biomarker Study.
• Data Synthesis and Integration: This effort focused on two major approaches.  One using a numerical version of a conceptual model that incorporated a number of different (yet connected) data sets. This “bottom up approach” model identifies specific fluxes and transformations among major P reservoirs within the STAs.  When integrated with existing data, key processes (settling, resuspension, etc.) were captured well with a better model-data match for EAV than for SAV. The “top down approach” model focused on two major controlling variables, internal load and flow. This model evaluated transect data and the effects of varying flows on outflow P concentrations. Model results suggest that internal loading and flow affected outflow P concentration dynamics. Further synthesis and integration beyond the P Flux study is being conducted in the Data Integration Study.

Reports and Publications

1. Villapando, R. and J. King, 2019, Appendix 5C-3: Evaluation of Phosphorus Forms. Flux, and Transformation Processes in the Stormwater Treatment Areas in Volume I 2019 South Florida Environmental Report.

2. DBE, 2018 Field and Supplemental Laboratory Support for Evaluating Phosphorus Sources, Flux, and Transformation Processes in the Stormwater Treatment Areas – Annual Report. Prepared by DB Environmental, Inc. under Agreement 4600003029-WO01 for South Florida Water Management District, West Palm Beach, FL.

3.  DBE, 2019, Field and Supplemental Laboratory Support for Evaluating Phosphorus Sources, Flux, and Transformation Processes in the Stormwater Treatment Areas – Annual Report. Prepared by DB Environmental, Inc. under Agreement 4600003029-WO01 for South Florida Water Management District, West Palm Beach, FL.

4.  Jerauld, M., et al., 2020, Internal phosphorus loading rate (iPLR) in a low-P stormwater treatment wetland, Ecological Engineering,Volume 156.

5. King, J., and R. Villapando, 2018, Appendix 5C-3: Evaluation of Phosphorus Forms. Flux, and Transformation Processes in the Stormwater Treatment Areas in Volume I 2018 South Florida Environmental Report.

6.  King, J. and R. Villapando, 2020, Chapter 5C: Evaluation of Phosphorus Forms. Flux, and Transformation Processes in the Stormwater Treatment Areas in Volume I 2020 South Florida Environmental Report.

7. DBE, 2017, Field and Supplemental Laboratory Support for Evaluating Phosphorus Sources, Flux, and Transformation Processes in the Stormwater Treatment Areas – Annual Report. Prepared by DB Environmental, Inc. under Agreement 4600003029-WO01 for South Florida Water Management District, West Palm Beach, FL.

8. University  of Florida, 2019 Evaluation of Soil Biogeochemical Properties Influencing Phosphorus Flux in the Everglades Stormwater Treatment Areas (STAs) Final Report. Submitted to South Florida Water Management District, West Palm Beach, FL.

9. Gann, D., M. Bernardo, and J. Richards, 2019, Detection and Differentiation of Submerged Aquatic and Emergent/Floating Marsh Vegetation using Remote Sensing Methods -- Spectral Separability from WorldView Data (Pilot Study). Report Submitted to the South Florida Water Management District, West Palm Beach, FL.

10. Florida International University, 2019, Settling and Entrainment Properties of STA Particulates Draft Final Report.  Prepared under Agreement No. 4600003032-WO02-9500006758 for South Florida Water Management District,. West Palm Beach, FL.

11 Evans, N., et al., 2019, Appendix 5C-4:  Effects of Abundant Faunal Species on Phosphorus Cycling in the Stormwater Treatment Area in Volume I 2019 South Florida Environmental Report.

12. Morrison, E., et al., Multiple biomarkers highlight the importance of water column processes in treatment wetland organic matter cycling. J Water Research.,  168: p. 115-153.

13. Juston, J. and B. Kadlec., Data-driven modeling of phosphorus (P) dynamics in low-P stormwater wetlands. Environmental Modelling & Software 118: p.:226-240.

14. Julian, Paul II., et al., Evaluation of nutrient stoichiometric relationships among ecosystem compartments of a subtropical treatment wetland.  Do we have “Redfield wetlands”?  Ecological Processes (2019) 8:20.  https://doi.org/10.1186/s13717-019-0172-x

15. Julian, Paul II., et al., Understanding stoichiometric mechanisms of nutrient retention in wetland macrophytes: stoichiometric homeostasis along a nutrient gradient in a subtropical wetland.  Oecologia (2020) 1-12. https://doi.org/10.1007/s00442-020-04722-9
 

Development of Operational Guidance for Flow Equalization Basins and Stormwater Treatment Area Regional Operation Plans

Description/Objective
Management of water flow into the STAs is critical to maintain Stormwater Treatment Areas (STAs) to achieve their objective.  This study developed tools and methods to support regional operation plans and guidance to optimize the use of Flow Equalization Basins (FEBs) and distribution of flow to the STAs to achieve the Water Quality Based Effluent Limits for Total Phosphorus (TP).

The study included three tasks:

1. Evaluate relevant information on STAs and conduct field tests to evaluate hydraulics and water quality.
2. Define values for model equations needed to simulate hydraulics, hydrology and operational control of STAs.
3. Develop local (STAs/FEBs) and regional operating strategies and rules and application of system optimization tools.

More information can be found here:

Detailed Study Plan

Activities Completed and Current Status

The study was completed in 2017.

Field measurements of waves propagated by inflow discharges to STA-3/4 Cell 3A were evaluated in relation to low, medium and high flow rates and water depth. Wave speeds and heights along flow paths were used to calculate vegetation resistance to flow. Maps of flow and resistance were created using the equations that described resistance at different flow rates. A total variation diminishing Lax-Friedrichs (TVDLF) method was used in a simple model to simulate flow-through hydraulic gradients of STA flow-ways based on real-time water elevation observations and flows. An inverse model (iModel) for Restoration Strategy Operational Protocol (RSOP) was developed to optimize flow rates along the Central Flow Path for proper flow attenuation and treatment with and without the FEB.

Results

Field tests improved water depth estimates and residence times in real-world applications leading to improved accuracy of physically based models of the STAs. The patterns of resistance produced from these applications reflected the general patterns of the vegetation distribution. The applications represented vegetation resistance, topography effects, resistance due to blockages, short-circuiting and turbulent behaviors. This information can assist project planning and design. The values in the model equations can determine if the flow behaves like porous-media flow or short-circuiting stream flow. The simple TVDLF method can forecast seven-day predictions of water depths under multiple management scenarios/flow regimes. At present this tool assists the management of the western flow-way of STA-3/4.

The iModel-RSOP was applied to four alternative scenarios of different FEB and Lake Okeechobee water delivery configurations. By rearranging flow to optimize TP removal, the iModel predicted lower TP concentration in discharge for all the scenarios compared to observed values. While the iModel results are encouraging, many difficulties were encountered to provide an accurate statistical prediction of TP concentration suggesting room for improvement.

Reports and Publications

1. Ali, A. 2015. Multi-objective operations of multi-wetland ecosystem: iModel applied to the Everglades restoration. Journal of Water Resources Planning and Management 141(9).
    
2. Ali, A. 2018. Appendix 5C-1 iModel for Restoration Strategy Operational Protocol Development. In: 2018 South Florida Environmental Report – Volume I, South Florida Water Management District, West Palm Beach, FL.
    
3. Lal, A. M. W. 2017. Mapping Vegetation-Resistance Parameters in Wetlands Using Generated Waves. Journal of Hydraulic Engineering 143(9).
    
4. Lal, A.M., M.Z. Moustafa and W. Wilcox, 2015. The use of discharge perturbations to understand in-situ vegetation resistance in wetlands. Water Resources Research 51:2477-2497; doi: 10.1002/2014WR015472.

Investigation of STA-3/4 Periphyton-based Stormwater Treatment Area Performance, Design and Operational Factors

Description/Objective
Investigate performance, design elements, operation and biogeochemical characteristics that have enabled the 100-acre PSTA Cell to achieve ultra-low outflow total phosphorus (TP) concentrations.

This study includes monitoring and surveys within the cell, as well as mesocosm and microcosm experiments that supplement these studies. Continuous TP measurements at inflow and outflow locations of the cell were also conducted.

More information can be found at:

Detailed Study Plan

Activities Completed and Current Status

The study was completed in 2018. Inflow and outflow monitoring is ongoing. 

Inflow and outflow concentrations of TP and flows into and out of the cell were monitored continuously. Grab surface water samples taken at the structures were analyzed for various P species. Other data measured periodically within the PSTA Cell included conductivity, pH, temperature, dissolved oxygen, chlorophyll a, turbidity, water depth, light and ion concentrations. Effects of pulse flows, season, time of day, water depth, inflow P concentration, flow rate and seepage on cell performance were evaluated. Enzyme assays were also conducted to evaluate the role of microbial activity. Sediment accrual and vegetation nutrient contents were also analyzed. Reports and manuscripts have been completed. Final analyses will be reported in the 2019 South Florida Environmental Report.

Results

The PSTA Cell achieved annual outflow flow-weighted mean TP concentrations ranging from 8 to 13 µg/L for the period of record (2008 to 2017). Pulse flow events and associated higher P loads had no adverse effect on treatment performance. Outflow TP concentrations were not affected by two different operational water depths (average of 0.39 m and 0.55 m) the time of day, or longer periods of constant moderate flow. Generally, lower outflow TP concentrations were observed during the wet season. The optimal inflow TP concentrations to the PSTA Cell is 22 µg/L or less, which achieved outflow concentration of 13 µg/L or lower. Lateral seepage through the levee between the Lower Submerged Aquatic Vegetation (SAV) Cell and the PSTA Cell was the major source of seepage. The accumulated sediment in the cell is low in P and chemically stable, resulting in sustained P removal performance.

Reports and Publications

1. James, R.T. 2015. Evaluation of Remote Phosphorus Analyzer Measurements and Hydrologic Conditions of the STA-3/4 Periphyton-based Stormwater Treatment Area. Technical Publication WR-2015-002. South Florida Water Management District, West Palm Beach, FL.
2. James, R.T. 2017. Analysis of Effects of Sustained Moderate Flow at the STA-3/4 Periphyton-based Stormwater Treatment Area. Technical Publication WR-2017-003. South Florida Water Management District, West Palm Beach, FL.
3. Zamorano, M. F. 2015. Effects of Pulse Flow Events on the STA-3/4 PSTA Cell's Phosphorus Treatment Performance. Technical Publication WR-2015-007. South Florida Water Management District, West Palm Beach, FL.
4. Zamorano, M. F. 2017. Influence of Seepage on the STA-3/4 PSTA Cell's Treatment Performance. Technical Publication WR-2017-004. South Florida Water Management District, West Palm Beach, Florida.
5. Zamorano, M. F., Grace, K., DeBusk, T., Piccone, T., Chimney, M., James, R. T., Zhao H., and Polatel, C. 2018. Appendix 5C-2: Investigation of Stormwater Treatment Area-3/4 Periphyton-based Stormwater Treatment Area Performance, Design, and Operational Factors. In: 2018 South Florida Environmental Report – Volume I, South Florida Water Management District, West Palm Beach, FL.
6. Zamorano, M., Piccone, T. and Chimney, M.J. 2018. Effects of short-duration hydraulic pulses on the treatment performance of a periphyton-based treatment wetland. ecological engineering 111(1): 69-77.
7. Zhao, H., T. Piccone, and M. Zamorano. 2015. STA-3/4 Periphyton-based Stormwater Treatment Area (PSTA) Cell Water and Total Phosphorus Budget Analyses. Technical Publication WR-2015-001. South Florida Water Management District, West Palm Beach, FL

Remote Environmental Sampling Technology: Evaluation of Sampling Methods for Total Phosphorus

Description/Objective

The objective of Restoration Strategies is to meet a stringent water quality based effluent limit (WQBEL) for phosphorus (P).  To assure that the WQBEL is reached, sampling and monitoring techniques must accurately measure total P (TP) concentrations The goal of this study is to determine the accuracy of the sampling and monitoring being used and to evaluate factors that may influence results.

More information can be found here:

Detailed Study Plan

Activities Completed and Current Status
The study was completed in 2017.

Various sampling methods were evaluated. Cameras were used to observe physical and biological disturbances that could affect sample quality. Methods were compared based on analytical results and sampling efficiency. Sample sets using different methods, including, Grab sampling, flow-proportional composite autosamplers (ACF) and discrete autosamplers based on time (ADT), were collected. These sets were compared with measurements from a remote phosphorus analyzer (RPA) deployed within the Stormwater Treatment Areas (STAs). Additionally, water quality sondes were deployed to measure conductivity, pH and turbidity.

Results
Data comparisons suggest that Grab and ADT collection methods are more reliable than ACF methods. Under some conditions, the ACF method may be biased and not meet the completeness (percent of acceptable samples collected based on planned number of samples to be collected) target of 90%. Completeness estimates differ, depending on whether they are based on time coverage and/or flow representation. Reverse, low or poorly defined flows interfere with ACF sampling and can lead to non-representative samples, particularly at sites with small water level differences from up to downstream. Data from the RPA method indicated a mid-day peak in TP concentrations.

Infrastructure and levees can function as habitat for wildlife and could be potential sources of TP. Some animals such as anhingas and turtles were observed interfering directly with sampling systems. Masses of submerged aquatic vegetation were also observed to accumulate often at the sample intake screens, but the influence of these events on TP results was not clear.

It is recommended that flow proportionality of autosamplers and comparison of ACF versus ADT at multiple locations be further investigated. The use of ACF method at structures with small water level differences from up to downstream should be discouraged. Evaluating the completeness of collection should be based on the amount of flow represented, rather than time. Also, methods for limiting wildlife impacts on surface water sampling should be studied further.

Publications

1. Rawlik, Peter 2017 Results from Project REST Remote Environmental Sampling Test. Technical Report WR-2017-002. South Florida Water Management District, West Palm Beach, FL.

Investigation of Rooted Floating Aquatic Vegetation in Stormwater Treatment Areas

Description/Objective

Rooted floating aquatic vegetation (rFAV) can make up a substantial amount of the vegetation in Stormwater Treatment Areas (STAs). They could be a valuable method of removing phosphorus (P) as they can grow in deep areas where emergent (EAV) and submergent aquatic vegetation (SAV) cannot. This study was to determine if rFAV enhance low-level P removal in back-end SAV communities of STAs.

The study included two phases:

Phase I of this study Evaluated P in the water column of rFAV and SAV patches to determine if differences exist.

Phase II of this study determined if Phase I findings were consistent among the STAs and then examined the mechanisms that account for the differences between patches.

More information can be found here:

Detailed Study Plan

Activities Completed and Status

The study was completed in 2018.

The spatial variability of water quality within rFAV and SAV patches and differences between rFAV and SAV patches over time were examined to determine factors that could explain patch differences [1, 2, 3]. Expanded sampling of additional patches verified findings from primary patches. This included measurements of water column pH, temperature, dissolved oxygen and conductivity. Soil physical properties and nutrients including porewater also were measured within each patch.

Results

Water column P concentrations were different for two of the three patches of rFAV plant species compared to nearby SAV patches [1, 2, 3]. Water column P concentrations in white water lily and American lotus patches were higher than in the nearby SAV patch, while water column P concentrations in a spatterdock patch were not significantly different from the nearby SAV patch. Soils in white water lily patches were lower in bulk density, higher in organic content and lower in calcium content compared to its paired SAV patch. This low bulk density soil may be prone to resuspension and could contribute to the higher particulate P concentrations in the water column. Dissolved oxygen and pH indicated greater aquatic metabolism in the SAV patch relative to the rFAV patch. Greater aquatic metabolism resulted from increased photosynthesis in the water column, which led to higher pH during the daylight hours. This higher pH may enhance co-precipitation of P with calcium resulting in more P removal. The soil from American lotus patches has a higher organic content than SAV patches but were similar otherwise.

Reports and Publications

1. Powers, M. 2018. Role of Rooted Floating Aquatic Vegetation, in 2018 South Florida Environmental Report – Volume I Chapter 5C: Restoration Strategies Science Plan Implementation (D. Ivanov, T. Piccone, T. James, and J. McBryan Eds.). South Florida Water Management District, West Palm Beach, FL.
2. Powers, M. 2019. Evaluation of the Role of Rooted Floating Aquatic Vegetation in STAs, in 2019South Florida Environmental Report – Volume I Chapter 5C: Restoration Strategies Science Plan Implementation (T. James, D. Ivanoff, T. Piccone, and J. King Eds.). South Florida Water Management District, West Palm Beach, FL.
3. DBE Environmental. 2018. Enhancing Vegetation-based Treatment in the STAs: Evaluations of Rooted Floating Aquatic Vegetation Deliverable #5b: Draft Technical Publication. Submitted to South Florida Water Management District; and Everglades Agricultural Area Environmental Protection; West Palm Beach Florida.

Evaluation of Inundation Depth and Duration Threshold for Cattail Sustainability

Cattail (Typha spp.) communities are an important component of Stormwater Treatment Areas (STAs). They remove particulate phosphorus (PP) by reducing water turbulence allowing the particles to settle. PP in moving water may be removed through colliding with and adhering to plant leaves. Soluble P is removed by algae attached to cattail leaves. Algae and PP can settle onto soils where they can be buried or degraded, releasing soluble forms of P. The soluble P may be taken up by roots and stored in rhizomes, roots, shoots or leaves of the plant.

Areas of cattail growing throughout STAs reduce water flow velocity and block the effects of winds from major storm events, thereby supporting aquatic vegetation.

Cattail are hardy plants, but they can decline or die if water levels are too deep for a long period.

Phase I of this study examined water levels and cattail stress within STA-3/4 Cell 2A through seasonal monitoring of water depths, cattail density, leaf elongation rate (LER), photosynthesis, and plant biomass with varying water depths [1,2]. Declines in any of these measures are an indicator of poorer cattail health.

Phase II of this study evaluated water depth and cattail stress in more controlled environments: half-acre test cells in which water levels were maintained at constant depths between 40 and 124 cm [3]. This part of the study provided evidence of the length of time that suboptimal (deep) water depths can be maintained without cattail communities collapsing. This information is useful for operational guidance of the STAs.

More information can be found here:

Detailed Study Plan

Activities Completed and Results

Phase I field monitoring was carried out from Water Year (WY: May through April) 2016 to WY2018 [1,2]. Cattail densities during the wet seasons were lower in the inflow region of STA-3/4 Cell 2A, where water levels are deeper compared to the outflow region of the cell. Cattail densities declined in the inflow region where water depths were greater than three feet (91 cm) for long periods of time. Leaf elongation rates were consistently higher in the inflow region. Photosynthetic rates and total live biomass were not different between the inflow and outflow regions. Throughout the STA cell, cattail biomass declined over time. The belowground biomass to leaf ratio declined over time in the inflow region indicating that the cattail population was stressed more in this region.

Phase II water depth experiments were carried out in the STA-1W Northern Test Cells (Figure 2 [3]). The Test Cells were refurbished in 2016 to improve soil and water control. Cattail seeds and seedling plants were added to the tests cells from May to June 2018. The plants fully matured by spring of 2019. The water depth experiments began July 1, 2019. One of five depth treatments was randomly assigned to three of the 15 cells: 1) 1.3 feet (40 cm, control), 2) 2.0 feet (61 cm, shallow), 3) 2.75 feet (84 cm, moderate), 4) 3.4 feet (104 cm, deep), and 5) 4.1 feet (124 cm, extremely deep]. Cattail density, water quality, LER, transpiration, water use efficiency and photosynthesis were measured monthly from July 2019 to late April 2020.

Final cattail biomass sampling and processing were completed late July 2020 [3].

All outflow P species except dissolved organic P (DOP) were significantly less than inflow P species for all treatments except the control cells. This exception is attributed to a lower amount of submerged aquatic vegetation and attached algae in the control cells due to the lower water levels. These lower water levels resulted in faster turnover of the water, compared to other treatments, reducing the ability of control cells to retain P.

Adult cattail densities declined over the 10-month period in all treatments, however, the densities were not significantly different from the control. Juvenile cattail density were significantly lower in the deep and extremely deep treatments (1.2 and 0.6 shoots/m2) compared to the control (3.2 shoots/m2) for the ten-month period, suggesting that long periods of deep water lead to reduced cattail density over time.

Cattails respond to increased water levels by increasing leaf length, which allows plants to maintain photosynthesis and activities related to gas exchange. These longer leaves explain the lack of significant differences among the treatments for photosynthetic rates, transpiration and water use efficiency values. Photosynthetic rates were significantly lower only for the extremely deep treatment compared to the control. While LER is important for the plant to survive increases in water level, excessive LER can lead to cattail collapse (see below). LER was significantly higher in cattail plants from the deeper treatments, with the higher LER measured during the active growing period (July-October).

Trade-off between density and weight in the cattail populations among the different water level treatments could in part explain the lack of significance of biomass change due to water depths in this study. However, there was a reduction in vegetative reproduction, as the juvenile density significantly decreased with increasing water depths.

The most significant difference among the treatments was noted at the end of the ten-month experiment. The faster LER in the deeper treatments resulted in less structural support of the leaves, causing substantial lodging (collapse) when water levels were lowered at the end of the study (Figure 2). Overall, cattail demonstrated tolerance at constant water depths ≥ 84 cm for ten months, however, cattail density (i.e., fewer juveniles and adults) and their ability to reproduce were significantly affected in the deep (104 cm) and extremely deep (124 cm) water level treatments and should be considered in the overall management of these systems.

Reports and Publications

1. Diaz, O., Appendix 5C-5: Evaluation of Inundation Depth and Duration Threshold for Cattail Sustainability., in South Florida Environmental Report – Volume I. 2018: South Florida Water Management District, West Palm Beach, FL.
2. Diaz, O. and K. Vaughan., Appendix 5C-2. Evaluation of Inundation Depth and Duration Threshold for Cattail Sustainability: In-Situ Study., in 2019 South Florida Environmental Report – Volume I. 2019: South Florida Water Management District, West Palm Beach, FL.
3. Diaz, O., Appendix 5C-1: Evaluation of Inundation Depth and Duration Threshold for Typha domingensis (Cattail) Sustainability: Test Cell Study., In 2022 South Florida Environmental Report – Volume I. 2022: South Florida Water Management District, West Palm Beach, FL.

Stormwater Treatment Area Water and Phosphorus Budget Improvements

Description/Objective

Water and phosphorus (P) budgets are important tools that can be used to understand the treatment performance of Stormwater Treatment Areas (STA). Accurate water budgets are critical to develop accurate P budgets. The objective of this study is to improve the annual water and P budgets for selected STA treatment cells or flow-ways.

The study includes two phases:

• Phase I – Evaluate the annual water budgets for STA-3/4 Cells 3A/3B; used as a test case. Evaluate sources of error and improve the annual water budgets [1].
• Phase II – Improve period of record (POR) flow data and water and P budgets for all cells of STA-3/4 [2, 4] and STA-2 Flow-ways 1-3 [3, 4].

More information can be found here:

Detailed Study Plan

Activities Completed and Current Status

The study was completed in 2020.

Phase I evaluation was completed in 2014 [1].

Phase II was completed in 2020 [2, 3, 4].

Period of record flow data for all cells of STA-3/4 [2] and STA-2 Flow-ways 1 to 3 [3] have been improved. Work to improve the South Florida Water Management District's Water Budget Tool was completed. Improved POR water and P budgets for STA-2 Flow-ways 1 to 3 were completed in 2017 [3] and all cells of STA-3/4 [2] were completed in 2018. Annual and long-term average annual flow-way and treatment cell Total P (TP) flow-weighted mean concentration (FWMC) in outflows, TP load retention percentage, and TP FWMC reduction percentage were calculated. The effect of annual hydraulic loading rate, annual phosphorus loading rate, annual average hydraulic residence time, inflow TP FWMC, and annual average water depth on annual outflow TP FWMC were evaluated.

Results

For STA-3/4 Cells 3A/3B, small differences in water levels across large culverts at mid-levees were the main source of error in annual cell-by-cell water budgets [2]. The budgets were greatly improved by updating flows between the upper and lower portions of the flow-way. Annual errors for the water budgets were reduced to 8% or less. Rainfall, evapotranspiration, change in storage and seepage were minor contributors to these cells' annual water budgets and the current methods for estimating these components were found to be acceptable for Phase II.

Annual water and P budgets were developed for STA-2 Flow-ways 1 to 3 for Water Year 2002 (WY2002) to WY2016, and for STA-3/4 Cells 1A, 1B, 2A, 2B for WY2006 to WY2017 and for STA-3/4 Cells 3A and 3B for WY2009 to WY2017 [4]. Improved POR flow and stage data for STA-2 and STA-3/4 inflow and outflow structures were used in the analyses. Rainfall, evapotranspiration, and seepage estimates were included. A new calibrated seepage coefficient (1.1 cfs/ft/mi) was used for the water budgets. These efforts resulted in more reliable water and P budgets and other performance estimates including annual and long-term average annual hydraulic retention times, hydraulic loading rates, P loading rates, and P reduction efficiency. For the six flow-ways studied, a higher frequency of low-level outflow TP annual FWMC was achieved when the annual hydraulic residence time was longer than 14 days, annual hydraulic loading rate was less than or equal to 3.5 cm day−1, the annual water depth was shallower than or equal to 0.65 meter, the annual P loading rate was smaller than or equal to 1.2 g m2 yr−1, or the annual inflow TP FWMC was less than or equal to 100 μg L−1. This study demonstrated that TP settling rate alone is not adequate to evaluate STA treatment performance especially when hydraulic loading rates differ. This study also helped improve understanding of the factors that affect the treatment performance of large-scale constructed wetland flow-ways which consistently retained TP and reduced TP concentrations over a long-term operational period.

Reports and Publications

1. Polatel, C., et al., 2014, Stormwater Treatment Area Water and Phosphorus Budget Improvements – Phase I: STA-3/4 Flow-ways 3A and 3B Water Budgets. Technical Publication WR-2014-004. . South Florida Water Management District, West Palm Beach, FL.
2. Zhao, H. and T. Piccone, 2019, Appendix 5C-5 Summary Report for Water and Total Phosphorus Budget and Performance Analysis for Stormwater Treatment Area 3/4. In: 2019 South Florida Environmental Report – Volume I. South Florida Water Management District, West Palm Beach, FL.
3. Zhao, H. and T. Piccone, 2018, Appendix 5C-6 Summary Report for Stormwater Treatment Area 2 Flow-ways 1, 2, and 3 Water and Total Phosphorus Budget Analyses. In: 2018 South Florida Environmental Report – Volume I. South Florida Water Management District, West Palm Beach, FL.
4. Zhao, H. and T. Piccone, Large Scale Constructed Wetlands for Phosphorus Removal, An Effective Nonpoint Source Pollution Treatment Technology. 145:. Ecological Engineering, 145.

Evaluation of Factors Contributing to the Formation of Floating Tussocks in the Stormwater Treatment Areas

Description/Objective

Floating tussocks (floating wetlands) in Stormwater Treatment Areas (STAs) can uproot Emergent Aquatic Vegetation (EAV), shade out Submerged Aquatic Vegetation (SAV), increase turbidity, block gates and other water control structures, and create short circuits. All these actions can reduce STA treatment performance.  This study evaluates factors that cause the formation of floating wetland areas in STAs and methods to determine current coverage of these floating areas.

The Study was completed in two phases:

Phase I of this study included four tasks:

1. A literature review of floating wetland types, buoyancy mechanisms, formation, and controls
2. A nomenclature of floating wetlands based on floating substrate, dominant plant community, size, and location
3. Classification maps of floating wetland communities in STA-1W Cell 2A and STA-2 Cell 7 using Unmanned Aircraft Systems (UAS)
4. Thermographic (infra-red) imagery to detect floating vs. emergent vegetation

Phase II of this study included three tasks:

1. Evaluation of relationships between occurrence and distribution of floating wetlands and environmental and management factors that could trigger floating wetland formation
2. A refined UAS methodology and workflow, used for surveys of all STA EAV cells
3. A Typha Wetland Buoyancy Model to evaluate root holding capacity vs increasing buoyancy as Typha are exposed to deeper water

More information can be found here:

Detailed Study Plan

Activities Completed and Status

All tasks are completed. Deliverables from Phase II: 2. UAS Assessment of STA Cells [5] and 3. Typha (cattail) Wetland Buoyancy Model [6] are in review.

Results

A review of the literature found a consistent terminology to describe the various types of floating vegetation does not exist. A nomenclature was developed, based on the presence or absence of floating substrate, horizontal connectivity, and dominant vegetation, to define floating emergent plant communities in the STAs [1].

An Unmanned Aircraft System (UAS) carrying multispectral camera was used to survey two EAV cells for floating wetland communities. Typha floating wetlands were found that were not observed in satellite imagery [2]. Floating wetlands were identified based on observed flattened vegetation, presence of fern seedlings, and nearby open seams. Thermographic (infra-red) imagery was attempted to improve the identification of these floating wetlands, but was difficult to interpret. However, midday thermographic images were useful and could complement associated images.

Historical data from the STAs were analyzed to identify significant predictors of floating wetland communities across EAV cells [3]. Results suggested a higher potential for floating vegetation occurs in and STAs: 1) if land had been farmed a short time before the STA was constructed, 2) if TP in the soil was low and 3) if water levels were exceedingly high (greater than 90% of the daily observed water levels). A buoyancy model based on this literature review and field measurements was developed in Phase II.

A refined method was developed to collect and process UAS data based on the previous survey [4]. Photos were collected using a Sentera AGX710 multispectral camera housed on a DJI Matrice 200 drone (Figure 1). This refinement of photo collection used a grid point sampling pattern at low altitudes (50-100 feet), improved accuracy during photo capture and reduced the amount of computer time needed to correct the photos. Photos could then be classified manually to determine vegetative community and its condition. This alternative approach was used to survey most of the STA cells with EAV, locating floating aquatic plants, floating organic vegetation and Typha lodging in a number of regions [5].

A buoyancy model was then developed to evaluate the effect of water levels and various plant and soil conditions on the probability that floating tussocks will occur [6]. This report is currently under review and completes this study.[SS1] 

                           Figure 1. Photo of a DJI Matrice 200 drone used in the mapping effort of the Tussock Study.

1. Clark, M. 2019. Proposed Floating Wetland Nomenclature. Phase I. Task 2b. Submitted by Soil and Water Science Department, University of Florida, Gainesville, FL, to South Florida Water Management District, West Palm Beach, FL.
2. Clark, M., and K. Glodzic. 2019. Phase I. Assessment of Floating Wetlands Coverage in STA EAV Cells. Task 3a Submitted by Soil and Water Science Department, University of Florida, Gainesville FL, to South Florida Water Management District, West Palm Beach, FL.
3. Clark, M., and K. Glodzic. 2020. Data Mining and Statistical Analysis of Environmental Factors Related to Floating Tussock Formation. Phase II Task 1 Submitted by Soil and Water Science Department, University of Florida, Gainesville FL, to South Florida Water Management District, West Palm Beach, FL.
4. Clark, M. and K. Glodzic. 2021. Evaluation of Factors Contributing to the Formation of Floating Tussocks in the STAs: Phase II Task 2a. Refined UAS Methodology and Workflow.  Submitted by the Soil and Water Science Department, University of Florida, Gainesville FL to South Florida Water Management District, West Palm Beach, FL
5. Clark, M., L. Griffiths, and K. Glodzik. 2022.  Evaluation of Factors Contributing to the Formation of Floating Tussocks in the STAs: Phase II Task 2b: UAS Assessment of Floating Wetland Formation in STA EAV Cells Submitted by the Soil and Water Science Department, University of Florida, Gainesville FL to South Florida Water Management District, West Palm Beach, FL.
6. Clark, M. 2022.  Evaluation of Factors Contributing to the Formation of Floating Tussocks in the STAs: Phase II Task 3: Typha Wetland Buoyancy Model. Submitted by the Soil and Water Science Department, University of Florida, Gainesville FL to South Florida Water Management District, West Palm Beach, FL.

Investigation of the Effects of Abundant Faunal Species on Phosphorus Cycling in the Everglades Stormwater Treatment Areas

Description/Objective

Aquatic fauna, fish and large invertebrates such as crayfish, may significantly affect phosphorus (P) cycling within Stormwater Treatment Areas (STAs). This study evaluates the potential of aquatic fauna to affect Total P reduction in STA surface waters. The study may provide management recommendations for aquatic fauna to improve STA treatment performance.

The study includes five tasks:

1. Estimate biomass of fish and aquatic invertebrates (STA-2) and aquatic faunal community composition (STA-1E, STA-1W and STA-3/4); analyzed P and nitrogen (N) storage in tissues of individuals of abundant fishes (STA-2) [1, 2]. This task is complete.
2. Estimate P excretion rates of selected abundant fishes to estimate contribution to STA P budgets [3]. Evaluated benthic (sediment foraging) fish to determine their contribution to water column nutrients through bioturbation (i.e., activities that resuspend soils, including particle disturbance, ingestion and defecation). This task is complete.
3. Use biomass and excretion estimates to calculate areal (per ha) P excretion by the entire aquatic faunal population in STA-2 (i.e., rates of P released to the water column via excretion, μg P/ha/ h). This task is ongoing.
4. Calibrate electrofishing through collection of non-native fish biomass by electrofishing compared to collection of these fish with block nets. Fieldwork is complete, analyses are ongoing.
5. Determine the effect of herbivory of SAV by large-bodied fishes using a 3-part enclosure and exclosure experiment focused primarily on the effects of the fish community as a whole and secondarily on the influence of invasive tilapia specifically. Fieldwork is complete, analyses are ongoing.

More information can be found here:

Work Plan

Activities Completed and Current Status

The STAs support a high biomass of fish and macroinvertebrates. By comparison, the biomass per unit area was approximately 2-15 times higher than in other Everglades regions [1, 2].

Ten of the most abundant fish species in STA-2 stored one metric ton of P and 3.7 metric tons of N within their body tissues, and excreted > 0.626 kg of P per hour and > 3.897 kg of N per hour [3,6]. Moreover, bioturbation by large fish was found to quickly and substantially increases the TP content of enclosures by 2 to 3 times [5,7]. These calculations demonstrate that storage, excretion, and bioturbation by fishes are important processes of P and N cycles within STA habitats [4]. Data from a recently completed herbivory experiment is being used to evaluate the influence of fish grazing of aquatic plants and periphyton on P reduction. Data from the P Flux, P Dynamics, Biomarker, and Water and P Budget Studies will be used to refine the current estimates and incorporate the effects of fish into P budgets for the STAs.

Reports and Publications

1. Evans, N., J. Trexler, and M. Cook, 2018, The Effects of Faunal Communities on Water Quality in the Everglades Stormwater Treatment Areas. In: 2018 South Florida Environmental Report – Volume I, Appendix 5C-3: Evaluation of Phosphorus Sources, Forms, Flux, and Transformation Processes in the Stormwater Treatment Areas,, O. Villapando and J. King, Editors.: South Florida Water Management District, West Palm Beach, FL.

2. Evans, N., et al., 2019, Appendix 5C-4: Effects of Abundant Faunal Species on Phosphorus Cycling in the Stormwater Treatment Areas. In 2019 South Florida Environmental Report – Volume I, . South Florida Water Management District, West Palm Beach, FL.

3. Barton, M., et al., 2020, Appendix 5C-1: Estimating Phosphorus and Nitrogen Excretion in Fishes in the Everglades Stormwater Treatment Areas In 2020 South Florida Environmental Report – Volume I, . South Florida Water Management District, West Palm Beach, FL.

4. Barton, M., et al., 2021, Appendix 5C: Investigation of the Effects of Abundant Aquatic Faunal Species on Phosphorus Cycling in the Stormwater Treatment Areas (Faunal Study) In 2021 South Florida Environmental Report – Volume I, . South Florida Water Management District, West Palm Beach, FL.

5. Goeke, J., et al., 2023, Appendix 5C: Investigation of the Effects of Abundant Aquatic Faunal Species on Phosphorus Cycling in the Stormwater Treatment Areas (Faunal Study) In 2023 South Florida Environmental Report – Volume I, . South Florida Water Management District, West Palm Beach, FL.

6. Barton, M., et al., 2023: Evaluation of the Impact of Aquatic Animal Excretion on Nutrient Recycling and Retention in Stormwater Treatment Wetlands. Ecological Engineering 197, 107104.

7. Goeke, J., et al., in review: Linking fish bioturbation to function and behavior traits in a eutrophic wetland: a preliminary analysis of fish contributions to internal nutrient loading. Freshwater Biology

Quantifying Phosphorus Uptake and Release from Periphyton and Phytoplankton Communities

Description/Objective

Periphyton (microbial communities of bacteria, algae, and fungi) grow on submerged aquatic plants surfaces, soils (epiphyton), or floating free in the water column (phytoplankton). These communities play an important role in phosphorus (P) cycling within the Everglades Stormwater Treatment Areas (STAs) through nutrient uptake, cellular growth, and senescence. The degree of periphyton nutrient cycling can affect P reduction in STAs, especially in outflow regions of the STA’s where P concentrations are very low (≤20 µg/L).

This study began as a substudy of the Phosphorus Flux study measuring enzyme activity in periphyton, floc, soils, and water column under various flow conditions and for EAV and SAV communities [3]. Phosphatase and N-acquisition enzymes were most active, while C-acquisition enzymes were least active. When dissolved organic P (DOP) in the surface water was above 10 μg/L, the phosphatase rates were low, suggesting P was not limiting. When DOP concentrations were <10 μg/L, phosphatase rates were greatly increased. The activity for N-acquisition enzyme was variable between EAV and SAV communities, but not along the flow-way. Using the ratio of N and P enzyme activity as surrogates for nutrient limiting conditions: both N- and P-limitation were found at the inflow and midflow sites. P-limitation only occurred at the outflows in all treatment cells. There were also indications that nutrient cycling differs between periphyton from the EAV and SAV communities under low-P conditions, suggesting there may be a complementary enzymatic response within a mixed marsh configuration that may enhance nutrient cycling.

This study quantifies periphyton processes of growth, senescence, and nutrient dynamics within the STAs. This information will indicate the significance of periphyton in P cycling and retention within the STAs. These results may provide important background information on periphyton P cycling processes that will improve our understanding of the factors that affect treatment performance at ultra-low TP concentrations.

There are three phases in this study:

Phase I was a literature review and analysis of periphyton-related research conducted in the STAs [1]. This phase is complete.

Phase II used lab and field experiments to evaluate periphyton processes and identify the analytical methodologies for use in the Phase III studies. This phase is complete.

Phase III uses advanced techniques to identify and evaluate periphyton community genetic structure and activity of nutrient cycling genes in the field. This phase is ongoing.

More information can be found here:

Project Summary

Activities Completed and Current Status

Phase I included two literature reviews that evaluated methodologies and results of periphyton studies with a focus on South Florida and the STAs [1, 2]. These reviews found that light intensity, temperature, and nutrient availability affect periphyton community structure. A few published rates on photosynthesis and growth, applicable to subtropical systems, were found. Approaches to measure periphyton community structure, productivity, decomposition, senescence, and nutrient uptake rates were reviewed. Information from these literature reviews will be applied to developing and implementing Phase III research studies. This phase is complete.

Phase II included bench top and field experiments. In the bench top study, the ability of periphyton to act upon bioavailable dissolved organic compounds in STA surface water were quantified in an incubation study completed in 2022. Metagenomics (which identifies the DNA of microbial species) of periphyton samples collected in the STAs were measured. The metagenomics results found numerous physiological processes (enzyme production, nutrient uptake, etc.) that could be tracked, potentially providing insight into the nutrient cycling potential of the epiphyte communities.

Phase III is a field-scale research study of periphyton metagenomics (DNA identification of periphyton microbial genes and species) and metatranscriptomics (quantifies active genes in microbes) to evaluate periphyton community responses to spatial and seasonal changes in water column nutrients and hydrology. This study began in December 2022, data analysis continues and is scheduled for completion in July, 2024.

Reports and Publications

1. Laughinghouse, H. D., Berthold, D. E, Barbosa, M., and Lefler, F. W, May 2019, A Review on Tropical and Subtropical Periphyton and Phytoplankton Processes and Methods of Quantification, draft. 
2. DB Environmental, 2021. Microbial Processes Affecting Phosphorus Removal and Retention in the Everglades Stormwater Treatment Areas.
3. Pietro, K. C., K. Inglett and A. Wright. 2023. Periphyton enzymatic activities in the water column along internal low-phosphorus nutrient gradients in the Everglades Stormwater Treatment Areas. Ecological Engineering 196: 107100.